Without five-time Ballon d’Or winner Lionel Messi, Jorge Sampaoli’s side suffered a 6-1 loss in Madrid, equalling their heaviest margin of defeat.
On a night when Javier Mascherano equalled Javier Zanetti’s Argentina caps record of 142, Sampaoli’s men were found woefully short all over the field and Isco grabbed his second goal seven minutes into the second half, with Thiago Alcantara making it 4-1 moments later.
The scoreline was made emphatic inside two minutes when substitute Iago Aspas scored shortly before Isco completed his hat-trick and left Argentina’s World Cup preparations in a sorry state.
The last time Argentina lost by five goals was a 6-1 drubbing to Bolivia in qualifying for the 2010 World Cup, and they were previously beaten by the same scoreline by Czechoslovakia in 1958.
Tuesday’s rout represented Spain’s biggest win over a side with a World Cup winto their name.